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. 2006 Aug 14;103(34):12747–12752. doi: 10.1073/pnas.0605333103

Fig. 4.

Fig. 4.

Variability in promoter configuration and regulation in the FoxO–Smad synexpression group. (A) A graphic comparison of the promoter regions of the FoxO–Smad synexpression genes is shown. The conserved FHBEs (green) surrounded by SBEs (orange) within 100 nt and the C/EBPβ binding elements (C/EBPβBE, blue) identified by computational sequence analysis are indicated. The track (black) indicates mouse sequence segments that are similar to its human ortholog. Percent identities of the segments ranged from 75% to 100%. Shaded boxes (orange) identify the functionally validated TGF-β responsive promoter regions. (B) Venn diagram depicting the overlap between FoxO-dependent and C/EBPβ-dependent TGF-β gene responses in HaCaT keratinocytes. (C) Effect of TGF-β on FoxO–Smad responsive genes in control and C/EBPβ-depleted HaCaT cells. Cells were incubated with or without TGF-β for 3 h. qRT-PCR was used to measure mRNA levels of the indicated genes. The fold increase of these levels by TGF-β is indicated. Data are mean ± SD (n = 3) of three independent experiments.