Skip to main content
. 2006 Aug 14;103(34):12849–12854. doi: 10.1073/pnas.0600427103

Fig. 4.

Fig. 4.

Osteoclast formation in TSHR or caTSHR overexpressing macrophages. (A) RAW-C3 cells stably transfected with empty vector, TSHR, or caTSHR were cultured with 100 ng/ml RANKL with or without human TSH (0.1–10 milliunits per ml) for 6 days. TRAP-positive osteoclasts were counted. ∗, P < 0.05 compares TSHR and caTSHR transformants with Empty-C3 cells at each concentration of TSH; +, P < 0.05, compares the effect of each concentration of TSH against zero-TSH in empty-C3 cells. (B) Bone marrow macrophages were transiently infected with either an empty retrovirus (empty) or a retrovirus containing either the TSHR or caTSHR construct. The infected cells were treated with RANKL (60 ng/ml) and M-CSF (30 ng/ml) for 6 days and examined for TRAP-positive osteoclast formation. ∗, P < 0.05, compares the effect of RANKL or RANKL plus TSH in TSHR-C3 and caTSHR-C3 cells versus empty-C3 cells; +, P < 0.05, compares the effect of RANKL plus TSH with that of RANKL alone (control) for each transformant.