Fig. 5.
PKA-mediated phosphorylation gradients in a 2D computational model of local DMNB–cAMP uncaging (DcAMP = 200 μm2/s). (A) AKAR phosphorylation images after global uncaging and then local uncaging (5-s UV activation restricted to red circle, timepoints indicated in B). p, proximal; d, distal. (B) Emission ratio time course from regions proximal and distal to the local uncaging site. (C) Model validation of propagation time (ΔtMAX) and gradient magnitude (ΔRP/ΔRD) for local DMNB–cAMP uncaging.