Fig. 7.

Intracellular blockage by NFA and SITS. (a) Native currents were activated in inside-out patches from dendritic/knob cilia of an OSN by 100 μM Ca at −50 mV. In the same patch, bath application of 1 mM NFA (gray trace, Upper) rapidly and reversibly blocked the current, whereas 2 mM SITS (gray trace, Lower) did not significantly block the current. Black traces in each image are Ca-activated Cl currents recorded before and after application of each blocker and show the stability of the recordings. (b) Ratios between native olfactory Ca-activated Cl currents measured in the presence and absence of SITS or NFA. (c) Comparison of average currents measured in mBest2-transfected HEK-293 cells activated by 5 μM intracellular free Ca²⁺ in control and with the intracellular addition of 300 μM NFA or 2 mM SITS.