Figure 1.

The expression of Wg dual receptors and their activities in S2 cells. One-step RT-PCR was performed to study the expression of Arr and DFz2 in mbn-2 cells and S2 cells (A). 0.1 μg RNA from each cell line served as the template, and primers for Arr and DFz1, 2, 3 and 4 (see Methods) were used for the RT-PCR reactions. (B) S2 cells were transfected with the indicated plasmids and the plasmids as described in Methods. Fold-activation values were measured relative to the levels of luciferase activity in cells transfected with empty vectors and normalized by Renilla luciferase activities. These values are plotted as a log function. Averages of the fold-activation are indicated above each column. All experiments were done in triplicate; error bars represent standard deviations.