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. 2006 Aug 28;103(36):13315–13320. doi: 10.1073/pnas.0605805103

Fig. 4.

Fig. 4.

Expression analysis and localization of CS. (A) Specificity of antibody raised against CS as evident from Western blot. Lanes: M, marker; A, SDS/PAGE of crude placental protein extracts; B, Western blot with anti-CS showing the specificity. (B) Developmental expression of CS. Lanes: a, 7-day-old placental protein; b, 14-day-old placental protein; c, 21-day-old placental protein; d, 28-day-old placental protein; e, 35-day-old placental protein; f, 42-day-old placental protein; g, 50-day-old placental protein (all high pungent C. frutescens); h, 28-day-old placental protein (low pungency genotype Arka Abhir C. annuum); i, 28-day-old placental protein (medium pungency genotype Arka Lohit C. annuum). (C) Immunolocalization of CS in placental tissues of C. frutescens. The immunolocalization studies indicated that CS is localized in peripheral cells of placenta of Capsicum fruits. (Ca) 15-day-old fruit. (Cb) 22-day-old fruit. (Cc) 30-day-old fruit. (Cd) 45-day-old fruit. pl, Placenta; pr, pericarp.