Fig. 5.

Amino acid sequence alignment of csy1 and acyl transferases. (a) Multiple sequence alignment of deduced amino acid sequence of csy1 gene with reported acyl transferases of Capsicum. pun1 and catf1, the reported acyltransferases speculated to be involved in regulation of pungency of Capsicum fruits, do not share significant homology with csy1. (b) Multiple sequence alignment of deduced amino acid sequence of csy1 gene with reported acyl transferases from Cucumis melo and Catharanthus roseus. The deduced amino acid sequence of csy1 is unique to Capsicum genus and is involved in capsaicin biosynthesis in Capsicum spp.