Expression analysis of csy1 gene. (a) Tissue and genotypes specific expression of csy1 through RT-PCR. Lanes: a, pericarp of high pungency genotype (C. frutescens); b, seed of high pungency genotype (C. frutescens); c, placenta of low pungency genotype (C. annuum var. Arka Abhir); d, placenta of medium pungency genotype (C. annuum var. Arka Lohit); e, placenta of high pungency genotype (C. frutescens). (b) Developmental expression of expression of csy1 in high pungency genotypes through RT-PCR.