Table 2.
Fractions | Activity, nmol/h | Recovery, % | Protein, mg/ml | Specific activity, units/mg of protein per h | Fold increase |
---|---|---|---|---|---|
Crude | 167 | – | 4.82 | 34.58 | – |
60% Ammonium sulphate fraction | 147.47 | 88.31 | 1.74 | 84.28 | 2.43 |
Gel-filtration | 129.87 | 77.77 | 0.94 | 1,137.95 | 39.8 |
AH Sepharose | 112.36 | 67.28 | 0.01 | 7,146.00 | 206.65 |