Fig. 1.

Generation of Chst5-null mice. (A) Structure of the targeting vector and generated mutant allele. Chst5 exon 2 (white box) including a single ORF (black box) was replaced with a neomycin-resistant gene (Neo, shown by a black arrow) in the targeting vector and generated mutant allele. The negative selection marker, DTA, and plasmid vector backbone, pBS, are shown by a black arrow and a gray line, respectively. (B) Normal and mutant alleles generated by homologous recombination with the targeting vector were detected by genomic PCR analysis using specific primers shown as black arrowheads in A. (C) RT-PCR analysis using primers indicated as white arrowheads in A also confirmed the presence and absence of Chst5 mRNA (indicated by an arrowhead) in the whole eyes of WT and Chst5-null mice, respectively.