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. 2006 Aug 28;103(36):13351–13356. doi: 10.1073/pnas.0605692103

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© 2006 by The National Academy of Sciences of the USA

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Fig. 2. — CARM1 inactivation impacts on the level and timing of expression of CCNE1. (A) mCCNE1 mRNA levels in exponentially growing CARM1^−/− or control (WT, CARM1^+/−) MEFs were determined by Q-RTPCR on total RNA. Histograms are the average of results obtained from two independent cell populations. (B) siRNA-mediated CARM1 knockdown leads to a decrease in CCNE1 mRNA level. Human U2Os cells were transfected either with a scrambled control siRNA or siRNA directed against human hCARM1 and analyzed 48 h later for hCCNE1, hCARM1, and hActin mRNA contents by Q-RTPCR. (C) Kinetics of expression of mCCNE1 mRNA after serum restimulation of G₀-arrested CARM1^−/− or control (WT, CARM1^+/−) MEFs. Serum-starved MEFs were incubated with high serum for the indicated time, and mCCNE1 mRNA levels were determined by Q-RTPCR.