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. 2006 Aug 28;103(36):13362–13367. doi: 10.1073/pnas.0602022103

Table 2.

Summary of mechanical parameters of cantilever beams at various stages of the biosensing analysis, in which protein attachment scheme 1 was used

Cantilever number LC, length of cantilever, μm WC, width, μm ωr, unloaded natural frequency, MHz Unloaded quality factor kC, unloaded linear spring constant, N/m kC+A, spring constant after composite protein attachment, N/m ΔωrA, measured change in resonant frequency after composite protein attachment, kHz Calculated minimum detectable frequency shift to measure composite protein attachment, kHz ΔωrB, measured change in resonant frequency after virus capture, kHz Calculated minimum detectable frequency shift to measure virus capture, kHz
SE_N1 3.1 1.7 2.407 7.28 0.0180 0.0160 −376 52 15 50
SE_N6 3.0 1.7 2.161 8.38 0.0173 0.0190 −236 47 −56 38
SE_N7 2.8 1.8 2.439 6.25 0.0142 0.0160 −87 64 −63 54
SE_N2a 2.8 1.6 2.237 5.88 0.0110 0.0160 −179 72 −27 45
SE_N3 2.9 1.6 2.246 6.32 0.0125 0.0162 −123 65 76 47
SE_N4a 2.5 1.6 3.242 13.05 0.0366 0.0281 −233 32 31 38
S5_N1a 3.4 1.5 2.633 6.11 0.0173 0.0220 −97 69 −67 51
S5_N2a 3.3 1.5 2.751 7.70 0.0232 0.0320 −195 57 −129* 35
S5_N4 3.3 1.4 2.146 4.89 0.0114 0.0197 −233 70 −66* 31
S5_N6a 3.1 1.4 2.930 6.87 0.0227 0.0330 −223 68 −112* 39
S5_N2b 4.3 1.5 1.227 7.95 0.0085 0.0094 112 25 −43 27
SE_N4b 3.1 1.8 1.831 8.05 0.0138 0.0165 82 49 −17 44
SE_N5 2.8 1.6 2.363 9.77 0.0201 0.0202 324 42 −66 49
S5_N5 3.3 1.4 1.767 7.23 0.0131 0.0202 55 39 −51 27
SE_N2b 3.2 1.7 1.933 10.17 0.0195 0.0193 108 38 −484* 41
S5_N1b 4.3 1.3 1.278 5.92 0.0062 0.0114 81 34 −71* 22
S5_N3 5.0 1.5 1.264 8.23 0.0091 0.0133 132 24 −58* 21
S5_N5b 4.5 1.5 1.256 6.28 0.0069 0.0123 633 32 −132* 45
S5_N6b 4.5 1.7 1.309 6.57 0.0076 0.0114 49 32 −104* 23
S5_N7 4.7 1.3 1.300 5.91 0.0072 0.0133 28 35 −60* 20

Lengths and widths were obtained through measurements using field emission scanning electron micrographs. Measured changes in resonant frequency after the virus capture step marked with asterisks are two times larger than the calculated minimum detectable frequency shift.