Table 1.

Kinetic analyses for GK and its mutants

Mutants	Protein conc., mg/ml	Vmax, mOD/min	Km for ATP, mM	S0.5 for glucose, mM	nH†
LGK2-WT	2.24	181.51 ± 6.78	0.14 ± 0.01	11.64 ± 0.83	1.40 ± 0.12
Y61S mutant	2.24	60.33 ± 3.83	0.08 ± 0.01	116.86 ± 7.46	1.23 ± 0.10
I159A mutant	2.24	23.32 ± 1.20	0.05 ± 0.01	95.16 ± 7.32	0.96 ± 0.04
A201R mutant	2.24	149.62 ± 8.50	0.20 ± 0.01	18.14 ± 0.26	0.76 ± 0.06
V203E mutant	2.24	39.26 ± 2.85	0.55 ± 0.07	201.23 ± 18.76	0.58 ± 0.04
V452S mutant	2.24	86.61 ± 1.78	0.23 ± 0.01	28.11 ± 0.73	0.96 ± 0.02

Data are means ± SEM for wild-type hLGK2 and hLGK2 mutants. The results are means of kinetic analyses of three independent experiments of wild-type hLGK2 and hLGK2 mutants.

^†nH is the Hill number.