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AT-III-based sensing of active heparin concentration. (a) Procedure for immobilizing AT-III to the sensor surface. (b) Dose–response curve for the AT-III sensor with heparin (●) and chondroitin sulfate (○), a carbohydrate that is structurally related to heparin but known not to interact with AT-III. Chondroitin sulfate data points are connected with a dashed line and heparin data points (shown as the average of two measurements ± 1 SD) are fitted to a Langmuir isotherm (solid line).
