Fig. 4.

Phosphorylation of T567 in ezrin is necessary for lamellipodium extension with EGF. (A) Time-lapse images of MTLn3 cells transfected with empty vector (Control) or vector encoding WT ezrin (Ezrin) or ezrin T567A (EzrinT567A) and treated with EGF (25 nM) are shown for the indicated times. See also Movies 4–6. Transfected cells (outlined in white) were identified by coexpression of GFP. Lamellipodium area was determined as in Fig. 1. (B) Mean ± SEM of lamellipodium area of six cells in three cell preparations is shown. (Scale bar: 10 μm.)