Fig. 5.

NIK activity is necessary for phosphorylation of ERM proteins by PDGF but not by thrombin. Representative immunoblots of lysates prepared from CCL39 fibroblasts infected with Ad or Ad-NIK-D152N and treated for the indicated times with PDGF (50 ng/ml) (A Upper and C) or thrombin (30 nM) (B Upper) are shown. Blots were probed with anti-pERM antibodies (A and B), antiphospho-ERK antibodies (C), or antiezrin antibodies (A–C). Data normalized from five (A Lower) and three (B Lower) cell preparations are expressed as the mean ± SEM of fold-change relative to abundance of pERM proteins in Ad-infected cells at time 0. The immunoblot in C is representative of five cell preparations.