Fig. 3.

Rdh5 expression is decreased in apc mutants. (A) Whole-mount in situ hybridization on wild-type or apc mutant larvae at 72 hpf for rdh5, prrdh1, prrdh2, rdh10, or rdh12. rdh5 and rdh12 expression are absent in apc mutants. (B) After whole-mount in situ hybridization on 96-hpf larvae with an antisense probe for rdh5 we performed histologic sectioning, which revealed the RPE-specific expression of rdh5. (C) HCT116 human colon cancer cells were transfected with empty vector or rdh5 and treated with retinol, and then RA was extracted and quantified by comparison with the internal extraction standard, TTNPB. Error bars represent standard deviation.