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PaMKK1 and PaASK1 act upstream of PaMpk1. Protein extracts (20 μg) from a wild-type strain grown for different durations (24, 36, and 48 h) on M2 medium and from the ΔPaMpk1, IDC404, and IDC118 mutants grown for 48 h were separated on gels and probed with antibodies that specifically detect the unphosphorylated form (anti-MAPK) and the phosphorylated form (anti-phospho-MAPK) of PaMpk1.
