Table 1.
PaMKK1 and PaMpk1 are required for production of C
| Donor strains | Recipient strains | C transmission |
|---|---|---|
| CG AS6-5* | NG AS6-5 | 30/30 |
| CG AS6-5* | AS6-5 IDC | 0/30 |
| CG AS6-5* | AS6-5 IDC | 0/30 |
| CG AS6-5* | AS6-5 IDC | 0/30 |
| CG AS6-5* | AS6-5ΔPaMpk1 | 0/30 |
| WT† | NG AS6-5 | 30/30 |
| IDC† | NG AS6-5 | 0/30 |
| IDC† | NG AS6-5 | 0/30 |
| IDC† | NG AS6-5 | 0/30 |
| ΔPaMpk1† | NG AS6-5 | 0/30 |
| IDC‡ | NG AS6-5 | 0/30 |
| IDC‡ | NG AS6-5 | 0/30 |
| ΔPaMpk1‡ | NG AS6-5 | 0/30 |
Shown are the number of successful contaminations of the indicated recipient strains by explants taken from the indicated donor strains (C donor) in experimental conditions identical to those of ref. 5. Cell fusion at the junction between recipient and donor is a natural phenomenon in P. anserina. With this system, up to 100% transmission is achieved in control experiments. None were observed in the PaMKK1 and PaMpk1 mutants.
*Explants were taken from the growing margin of the CG AS6-5 culture.
†Explants were taken from the stationary phase area of the indicated cultures, i.e., at the center of the thallus.
‡Explants were taken from cultures that were previously infected by a CG AS6-5 culture.