Fig. 3.

AdPC1/3 transduction enhances glucose-stimulated insulin secretion and survival of isolated mouse islets. (A) Glucose-stimulated insulin secretion in mouse islets 72 h after transduction with AdPC1/3 (moi = 100). Area under curve (AUC; Inset) was determined for the entire curve normalized for baseline. Data were analyzed with a one-tailed t test; n = 4 with two independent replicates per experiment. (B and C) AdPC1/3-transduced islets are protected against 24-h IL-1β treatment. (B) Merged image of live (green) and dead (red) cells in whole mouse islets treated with AdPC1/3 or Ad5lacZ (moi = 10) and with or without IL-1β (50 units/ml). (Scale bar: 200 μm.) (C) Quantification of cell death in islets treated with IL-1β, reported as average red fluorescence intensity normalized for the islet area. Data were collected from 6–15 individual islets per condition for two independent experiments and analyzed by using one-way ANOVA. ∗, P < 0.05; ∗ ∗, P < 0.01.