Fig. 2.
Apoptosis and viability of L3055 cells in response to DA. (a) L3055 cells containing empty transfection vector (L3/VC) or containing the bcl-2 gene (L3/Bcl-2) were seeded at 2.5 × 105 per milliliter and treated with or without DA at the concentrations indicated for 6 h. Cells were dual-stained with PI and caspase substrate phiphilux and analyzed by flow cytometry. Dot plots are representative of three experiments showing the percentage of viable (caspaselo/PIlo), early apoptotic (caspasehi/PIlo), late apoptotic (caspasehi/PIhi), and necrotic (caspaselo/PIhi) cells. (b) Viability of (wild-type) WTL3055, L3/VC, L3/Bcl-2, or KHM-2B (2.5 × 105 per milliliter) cells in response to prolonged (24-h) DA exposure as assessed by PI staining. (c) Viability of resting PBMC or tonsil B cells (5 × 105 per milliliter) in response to DA for 24 h assessed as in a. (Upper) PBMC. (Lower) Percentage (of control) viable cells (caspaselo/PIlo) for B cells from three individual tonsils.