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EMSA of RovA with Y. pestis promoters. Randomly 32P-labeled rovA [+1 kb to −20 bp from start codon (SC)], inv (+500 to −20 bp from SC), gyrB (+500 to −20 bp from SC), psaA (+500 to −20 bp from SC), and psaE (+400 to −20 bp from SC) were incubated with the indicated concentrations of purified RovA in the presence of competitor DNA (1 μg of salmon sperm) as described in Materials and Methods.
