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. 2005 Jun 23;360(1458):1119–1132. doi: 10.1098/rstb.2005.1656

Figure 2.

Figure 2

Minichromosome maintenance protein MCM3 is not concentrated at sites of DNA replication whereas replication protein A(RPA) is. Panel (a) shows a nucleus of a Xenopus cultured cell pulse labelled to reveal newly replicated DNA (red) and immunostained for MCM3 (green). Panel (b) shows part of a Xenopus sperm nucleus replicating in Xenopus egg extract stained for newly synthesized DNA (red) and MCM3 (green). Panel (c) shows part of a Xenopus sperm nucleus replicating in Xenopus egg extract under identical conditions to those used in panel (b), but stained for newly synthesized DNA (red) and RPA (green) for comparison because RPA is known to be located at replication forks. MCM3 staining (green) is not co-localized with newly replicated DNA (red) in panel (a) and panel (b) whereas RPA staining is co-localized, producing yellow spots in panel (c). Reproduced with permission from Laskey & Madine (2003).