Figure 4.

Effects of BCNU on redox status and AP-1 activity. (a) Human T lineage cells (Molt-4) were treated with the glutathione reductase inhibitor BCNU at the indicated concentrations. After 3 h the cells were harvested, intracellular concentrations of total glutathione and glutathione disulfide were determined, and reduced glutathione (GSH) was calculated from the difference. (b) Molt-4 cells were transiently transfected with a CAT reporter construct driven by the thymidine kinase promoter under the control of 6 AP-1 binding sites. One day later the cells were treated with BCNU at the indicated concentrations and with or without TPA (10 ng ml⁻¹). Transactivation was determined after 36 h by CAT assay. (For other details see Galter et al. 1994.)