Voltage and NADPH-dependence of electron currents. Electron currents were evoked by bath application of increasing concentrations of NADPH, and voltage ramps ranging from −120 to 200 mV were applied to establish the current to voltage relationship of the electron current. (a) Steady-state amplitude of electron current at −40 mV. (b) Ramp currents recorded at intermediate and saturating concentrations of NADPH. At low NADPH concentration, the electron currents become voltage independent at negative voltages. Reproduced from Petheö & Demaurex, ‘Voltage- and NADPH-dependence of electron currents generated by the phagocytic NADPH oxidase’, The Biochemical Journal, 2005, vol. 388, pp. 485–491, by copyright permission of Portland Press Ltd.