TABLE 2.

Estimates of nucleotide variation

Parameter	Value	Frequency
No. of loci screened	66
Total length of amplicons in base pairs	25,138
No. of bases of sequence screened	1,397,461
No. of all sequence variants	1,145	1/22 bp
No. of indels	57	1/441 bp
No. of nucleotide substitutions	1,088	1/23 bp
No. of biallelic nucleotide substitutions	1,055	1/24 bp
Transitions/transversions (TI/TV) ratio	1.48
No. of triallelic nucleotide substitutions	30	1/838 bp
No. of tetra-allelic nucleotide substitutions	3	1/8379 bp
Nucleotide polymorphism (θ × 10−3), mean	11.5
Nucleotide polymorphism (θ × 10−3), coding region	10.1
Nucleotide polymorphism (θ × 10−3), nonsynonymous level of diversity	6.3
Nucleotide polymorphism (θ × 10−3), synonymous level of diversity	14.9
Nucleotide polymorphism (θ × 10−3), noncoding region	11.9
Nucleotide polymorphism ratio, coding/noncoding	0.85
Nucleotide polymorphism ratio, nonsynonymous/synonymous	0.42
Nucleotide diversity (π × 10−3), mean	14.6
Nucleotide diversity (π × 10−3), coding region	11.2
Nucleotide diversity (π × 10−3), nonsynonymous level of diversity	9.3
Nucleotide diversity (π × 10−3), synonymous level of diversity	18.0
Nucleotide diversity (π × 10−3), noncoding region	15.8
Nucleotide diversity ratio, coding/noncoding	0.71
Nucleotide diversity ratio, nonsynonymous/synonymous	0.52
Tajima's D, mean	0.5
Tajima's D, coding region	0.2
Tajima's D, noncoding region	0.9