TABLE 1.
Identification of differentially expressed genes
No assumption of equal variance
|
Assume equal variance
|
No. of probe setsa
|
|||||||
---|---|---|---|---|---|---|---|---|---|
FDR = 0.05 | FDR = 0.10 | FDR = 0.25 | FDR = 0.05 | FDR = 0.10 | FDR = 0.25 | % probe setsa | B73 > Mo17 | Mo17 > B73 | |
B73 vs. Mo17 | |||||||||
11-day seedling | 1633 | 3284 | 7400 | 3135 | 4784 | 8726 | 9.3 | 767 | 866 |
Immature ear | 740 | 1732 | 4749 | 2031 | 3209 | 6269 | 4.1 | 442 | 298 |
19 DAP embryo | 3264 | 5355 | 8907 | 5108 | 6786 | 9935 | 18.4 | 1437 | 1827 |
B73 × Mo17 vs. Mo17 × B73 | |||||||||
11-day seedling | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Immature ear | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
19 DAP embryo | 0 | 0 | 0 | 0 | 0 | 214 | 0 | 0 | 0 |
Analysis of differential expression in B73 relative to Mo17 or B73 × Mo17 relative to Mo17 × B73. Differentially expressed genes were identified using a one-way ANOVA to test GC-RMA values. Tests were performed with and without an assumption of equal variance with a Benjamini–Hochberg multiple testing correction and varying false discovery rates (FDRs).
These calculations are based on the list of differentially expressed genes identified using no assumption of equal variance and an FDR = 0.05.