TABLE 3.
Primers (F and R) and probes (a, b, or c) used for Taqman probing and minisequencing
| SNP | Polymorphism | Primer/probe sequence (5′-3′) |
|---|---|---|
| GHR snp1 | F279Y | F CTTTGGAATACTTGGGCTAG |
| R CACTTCACTCAGGATTCAC | ||
| AGTGACATTATATTTACTCATATa | ||
| AGTGACATTATTTTTACTCATAb | ||
| GHR snp2 | N528T | F AGTGTGACACGCACCCAGAA |
| R CCCAGCTGTAGTGGTAAGGCTTT | ||
| TCATCGTGGACAACGCTTACTTCTGCa | ||
| TTCATCGTGGACACCGCTTACTTCTGb | ||
| GHR snp3 | A541S | F AGTGTGACACGCACCCAGAA |
| R CCACATGATGAGAGAAACTC | ||
| GAGGTAGACGCCAAAAAGTACATTc | ||
| GHR snp4 | S555G | F AGTGTGACACGCACCCAGAA |
| R CCACATGATGAGAGAAACTC | ||
| AGCTAACTTCATCGTGGACAc | ||
| PRLR snp5 | S18N | F TGCAGCATCTAGAGTGGTTTTCA |
| R GAACAAGACAGTCTCTTACTTACCATTCA | ||
| ACTTTTTCTCAACGTCAGCCTTa | ||
| TACTTTTTCTCAGTGTCAGCCTTb | ||
| PRLR snp6 | L186P | F GACCTACATACTGGCTTCTCTGC |
| R GCAGATTTCAGGCAGAATCC | ||
| CCTCCCTAGACTCATTTTACTCc |
The polymorphic nucleotide in Taqman probes is presented in boldface type.
a
The sequence of FAM-labeled Taqman probe.
b
The sequence of VIC-labeled Taqman probe.
c
The sequence of primer extension oligo.