Figure 6.—

Analysis of polymorphisms in the un-24–het-6 region in 20 N. crassa strains. Eight different markers in and around In(het-6) were assayed for OR- and PA-specific PCR–RFLP patterns using primer pairs and restriction enzymes listed in Table 4. Inversion type was determined by PCR with primers Gene7b-P1/cys3-P4 (OR) and Gene7b-P1/6J-P11 (PA), corresponding to brk-1, or with Gene2-P3/6J-P11 (OR) or Gene2-P3/Gene 7a-P5 (PA), corresponding to brk-2 (see Figure 4 for primer locations). Strains RLM58-18 and 74OR23-1V were used as reference PA and OR strains, respectively. Correlation between each marker and the genotype at het-6 was calculated with the Fisher exact test.