TABLE 4.

Diagnostic markers for allele typing in the un-24/het-6 region

			Approximate fragment size (kbp)
Genetic marker	Primer pair	Restriction enzyme	OR	PA
Gene2	Gene2-P1 and Gene2-P2	KpnI	0.7, 0.2	0.9
un-24	6J-P11 and 6J-P6	PvuI	0.75, 0.75	1.5
Gene4-1	Gene4-P1 and Gene4-P2	XhoI	0.8, 0.7	1.5
Gene4-2	Gene4-P1 and Gene4-P2	KpnI	1.1, 0.4	0.7, 0.4. 0.4
Gene5	Gene5-P1 and Gene5-P2	NcoI	2.3, 1.0, 0.3	2.3, 1.3, 0.4
brk1-1	6V-P5 and 6J-P11	None	—	7.5
brk1-2	Gene7b-P1 and cys3-P4	None	4.5	—
brk1-3	Gene7b-P1 and 6J-P11	None	—	3.5
brk2-1	Gene2-P3 and 6J-inv2	None	1.8	—
brk2-2	Gene2-P3 and Gene7a-P5	None	—	2.3
brk2-3	Gene2-P1 and 6J-P11	None	5.6	—
het-6	6V-P3 and 6V-P5	MboI	0.5, 0.5	1
Gene9-1	Gene9-P5 and Gene9-P6	BglII	1.9, 0.2	2.1
Gene9-2	Gene9-P5 and Gene9-P6	HincII	0.8, 0.7, 0.5	1.3, 0.7

OR- and PA-specific fragments were amplified with primer pairs (see Table 2 for primer sequence and Figures 4 and 6 for locations) and, where noted, subjected to restriction enzyme digests.