TABLE 1.
SNPs identified and tested in this study
| SNP | Primer A | Primer B | No. of SNPs in amplicon | B6/BALB allele | CAST allele |
|---|---|---|---|---|---|
| SNP-846 | 5′-GGCTAAGCCATCACTTATCC-3′ | 5′-AAGATTCCTAACTGTTCTGGG-3′ | 3 (all in dbSNP) | GCT | TTC |
| SNP-828 | 5′-CCATGGAGATGATGACAAGC-3′ | 5′-GGCCTACAAAGCGACTTCC-3′ | 1 novel | A | G |
| LeeSNPG-Ia | 5′-GCTTGGTTCGTCTATCTTGTGGG-3′ | 5′-CCAGAGTCTGATGTAACGGAGG-3′ | 1 | Not digested by ScrFI | Digested by ScrFI |
| SNP-843 | 5′-TGTTTCCATGCCTCAGAAGC-3′ | 5′-GAACCACACTGCTTAACTAGC-3′ | 3 novel 2 in dbSNP | TACCA | ACTAG |
SNPs were genotyped by PCR amplification of genomic DNA using the primers indicated followed by sequencing.
a
This assay was published in Stavropoulos et al. (2001). To genotype this SNP, genomic DNA was PCR amplified with the primers indicated and digested with ScrFI, and products were separated by gel electrophoresis.