TABLE 3.

Rates of recombination between polymorphic and dimorphic EMS-induced a1 alleles

F1 heterozygote class			No. isolated		No. confirmeda		Population size	Recombination rate (cM)c
		Cross	F1 allele combination	Cl etb	Cl Etb	Cl et			Cl Et
Polymorphic	5	a1-3849-9 Sh2 et/a1-3845-5 Sh2 Et	5	441	2	1d	222,537	0.0013 ± 0.00008
Dimorphic	6	a1-3849-9 Sh2 et/a1-3849-5 Sh2 et	20	208	17	0	180,483	0.0094 ± 0.0002

^aIntragenic recombinants were confirmed by a combination of genetic crosses and molecular analysis (e.g., genotyping for the a1 pollen parent).

^bCl et, colored etched kernels; Cl Et, colored nonetched kernels.

^cCalculated as (no. confirmed Cl et + Cl Et)/population size × 100. Standard errors were calculated as described in Table 2, footnote g.

^dTwo of the isolated Cl et candidates were PCR-positive for the pollen parent a1 allele. One was tested but not confirmed by genetic cross and the other died at the seedling stage. For the most conservative estimate of intragenic recombination, it is assumed that the dead seedling would have been confirmed and, therefore, there is one Cl et recombinant.