TABLE 1.
Deletion size in bpa | IDb | Directionb | Single-male crosses | A | B | C | D | A′ | B′ | C′ | D′ |
---|---|---|---|---|---|---|---|---|---|---|---|
0 | 50C | None | 85 | 2,570 | 1,792 | 1,591 | 2,640 | 433 | 254 | 209 | 422 |
3,840 | 49 | Proximal | 79 | 1,510 | 1,532 | 1,480 | 2,040 | 91 | 37 | 35 | 78 |
6,848 | 81 | Proximal | 47 | 1,549 | 1,082 | 1,050 | 1,937 | 99 | 102 | 88 | 147 |
7,582 | 85 | Proximal | 75 | 1,256 | 1,074 | 1,179 | 1,630 | 99 | 118 | 100 | 108 |
10,160 | 102 | Proximal | 80 | 2c | 2,158 | 2,236 | 3,040 | 26 | 23 | 24 | 44 |
10,931 | 45 | Proximal | 47 | 28c | 827 | 439 | 1,089 | 25 | 88 | 57 | 125 |
42,645 | 36 | Distal | 114 | 4c | 2,915 | 2,503 | 3,690 | 4 | 357 | 305 | 195 |
210,463 | 38 | Distal | 239 | 0 | 7,205 | 6,030 | 9,067 | 0 | 666 | 500 | 394 |
Deletion lengths are based on a standard reference sequence and will vary slightly with stock.
Abbreviations for deletion chromosome names and directions are as reported (Preston et al. 1996). Standard nomenclature would be, e.g., Df(2R)50C-49.
The chromosomes with 10-, 11-, 43-, and 210-kb deletions are missing one or more essential genes encompassed by the deletion. Therefore, most individuals in category A do not survive from those crosses. A few exceptions were expected, however, resulting from coincidental occurrence of both gap repair and P-element transposition to place a copy of the P{w*} element on one of the other paternal chromosomes. We tested 20 of these exceptions by PCR similar to the test done for category A′ and confirmed that gap repair had occurred in each case.