TABLE 1.

DNA sequences of the mutagenic primers used to generate the mutations in this study

Primer name	Primer sequence
Mutagenic primer T18A	5′-CTCGTAGTCACAGTAGGCGCGGGTCTCTGG-3′
Mutagenic primer T18E	5′-CTCGTAGTCACAGTACTCGCGGGTCTCTGGACG-3′
Mutagenic primer S24E	5′-CATGCACTCGTTGACCTCCTCGTAGTCACAGTA-3′
Mutagenic primer E27Q	5′-CACGCCCTCCATGCACTGGTTGACGCTCTC-3′
Mutagenic primer T63A	5′-GCTAATGTCCACCATCGCGTCGATGAAATC-3′
Mutagenic primer T18A S24A	5′-CTCCATGCACTCGTTGACGGCCTCGTAGTCACAGTAGGCG-3′
Mutagenic primer T18E S24E	5′-CTCCATGCACTCGTTGACCTCCTCGTAGTCACAGTACTCG-3′

The sequences are in the antisense orientation. The antisense sequences for the amino-acid substitutions are underlined.