TABLE 2.
Rescue of the mutant wing phenotype of e(r)p1 rhd1-12 males
Wing phenotypes
|
|||||
---|---|---|---|---|---|
Transgenea | Male genotype | 1 | 2 | 3 | 4 |
e(r)+ | y1 w67c23 e(r)p1 rhd1-12; transgene/+ | 36 | 1135 | 0 | 0 |
y1 w67c23 e(r)p1 rhd1-12; +/+ | 28 | 8 | 1065 | 58 | |
T18A | y1 w67c23 e(r)p1 rhd1-12; transgene/+ | 16 | 401 | 24 | 0 |
y1 w67c23 e(r)p1 rhd1-12; +/+ | 8 | 28 | 322 | 26 | |
T18E | y1 w67c23 e(r)p1 rhd1-12; transgene/+ | 27 | 119 | 0 | 0 |
y1 w67c23 e(r)p1 rhd1-12; +/+ | 32 | 12 | 102 | 11 | |
S24E | y1 w67c23 e(r)p1 rhd1-12; transgene/+ | 19 | 615 | 2 | 0 |
y1 w67c23 e(r)p1 rhd1-12; +/+ | 9 | 6 | 685 | 29 | |
E27Q | y1 w67c23 e(r)p1 rhd1-12; transgene/+ | 27 | 92 | 0 | 0 |
y1 w67c23 e(r)p1 rhd1-12; +/+ | 22 | 2 | 83 | 0 | |
T63A | y1 w67c23 e(r)p1 rhd1-12; transgene/+ | 14 | 187 | 0 | 0 |
y1 w67c23 e(r)p1 rhd1-12; +/+ | 9 | 0 | 183 | 0 | |
T63E | y1 w67c23 e(r)p1 rhd1-12; transgene/+ | 9 | 113 | 0 | 0 |
y1 w67c23 e(r)p1 rhd1-12; +/+ | 12 | 2 | 125 | 6 | |
T18A-S24A | y1 w67c23 e(r)p1 rhd1-12; transgene/+ | 3 | 0 | 46 | 0 |
y1 w67c23 e(r)p1 rhd1-12; +/+ | 1 | 0 | 39 | 0 | |
T18E-S24E | y1 w67c23 e(r)p1 rhd1-12; transgene/+ | 54 | 47 | 517 | 6 |
y1 w67c23 e(r)p1 rhd1-12; +/+ | 51 | 35 | 511 | 10 | |
T18A-T63A | y1 w67c23 e(r)p1 rhd1-12; transgene/+ | 27 | 222 | 5 | 0 |
y1 w67c23 e(r)p1 rhd1-12; +/+ | 24 | 6 | 200 | 0 | |
T18E-T63E | y1 w67c23 e(r)p1 rhd1-12; transgene/+ | 1 | 76 | 0 | 0 |
y1 w67c23 e(r)p1 rhd1-12; +/+ | 2 | 0 | 34 | 0 | |
S24E-T63E | y1 w67c23 e(r)p1 rhd1-12; transgene/+ | 13 | 104 | 6 | 0 |
y1 w67c23 e(r)p1 rhd1-12; +/+ | 8 | 0 | 94 | 2 | |
T18A-S24A-T63A | y1 w67c23 e(r)p1 rhd1-12; transgene/+ | 8 | 2 | 106 | 1 |
y1 w67c23 e(r)p1 rhd1-12; +/+ | 7 | 0 | 134 | 0 | |
T18E-S24E-T63E | y1 w67c23 e(r)p1 rhd1-12; transgene/+ | 30 | 1 | 180 | 6 |
y1 w67c23 e(r)p1 rhd1-12; +/+ | 33 | 0 | 219 | 8 |
The crosses used to generate these flies are explained in materials and methods. Wing phenotypes were determined using the scale shown in Figure 1.
Data collected from one insert per construct: e(r)+, T18A, T18E, S24E, T18E-T63E; data collected from two inserts per construct: E27Q, T63A, T63E, T18A-S24A, T18A-S24A-T63A; data collected from three inserts per construct: S24E-T63E, T18E-S24E-T63E; data collected from four inserts per construct: T18E-S24E, T18A-T63A.