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. 2006 Sep;174(1):215–227. doi: 10.1534/genetics.106.058362

Figure 5.—

Figure 5.—

Δget3 rescues phenotypes of Δget2 and Δget1 cells. (A) Representative sporulated cells displaying wild-type or defective terminal sporulation phenotypes. Synchronously sporulated SK1 cells were fixed 36 hr after shift to sporulation medium (SPM) and nuclei were stained with DAPI. Nomarski (left) and DAPI (right) images representative of the indicated terminal (36 hr) sporulation phenotypes are shown. (B) Δget3 rescues terminal sporulation defects displayed by Δget2 cells. Synchronously sporulated wild-type (WT), Δget3, Δget2, and Δget3 Δget2 homozygous diploid yeast (SK1 background) were stained with DAPI, and cells falling into each of the four categories shown in A were counted. The average percentage of each phenotype is graphed with error bars depicting the standard deviation over three separate experiments. At least 200 cells were counted for each strain per experiment. (C) Δget3 suppresses the HU sensitivity of Δget2 and Δget1 cells. Wild-type (WT), Δget3, Δget1, Δget2, and double-mutant strains were grown to log phase and then serially diluted and plated to YPD or media containing 150 mm hydroxyurea at 25° for 2 days.