TABLE 2.
Suppressors of npl4-1
| Gene | Cellular activity | Allele(s) | |
|---|---|---|---|
| ERAD | |||
| YMR264W | CUE1a,c | Docking of Ubc7 in ERAD | Transposon after AA72, EMS (5) |
| YMR022W | QRI8/UBC7a,c | Ubiquitin-conjugation to ERAD substrates | EMS (16) |
| Secretion | |||
| YNR006W | VPS27 | Sorting of ubiquitinated proteins in MVB pathway | Transposon after AA260 |
| YLR119W | SRN2/VPS37 | Sorting of ubiquitinated proteins in MVB pathway | Transposon after AA44 |
| YDR069C | DOA4c | Deubiquitination in endosome to vacuole transport | EMS (8) |
| YER151C | UBP3c | Deubiquitination in vesicle transport | EMS (2) |
| Transcription/RNA modulation | |||
| YKL020C | SPT23b | Activation of OLE1 transcription | Transposon after AA710 |
| YLR223C | IFH1 | Chromatin assembly and silencing | Transposon after AA155 |
| YBR055C | PRP6 | Pre-mRNA splicing (U4/U6-U5 snRNP) | Transposon after AA793 |
| YMR125W | STO1/CBP80 | Nuclear mRNA cap-binding protein | Transposons (2) in noncoding regions |
| YDL100C | GET3c | Homolog of bacterial arsenite transporter | Transposon after AA215 |
Suppressors generated by EMS or transposon mutagenesis are listed by functional class. Several were isolated multiple times, as indicated. For mutants isolated from the transposon screen, the integration site is listed.
a
Previously published in Hitchcock et al. (2003).
b
Previously published in Hitchcock et al. (2001).
c
Null allele was tested and also found to rescue growth of npl4-1.