TABLE 2.
Mutations in nos-3, spat-3, or rpn-10 can suppress par-2 lethality
| Genotype | Embryonic viability at 20° (%)a | Embryonic viability at 25° (%)a |
|---|---|---|
| Wild type | 99.8 ± 0.0 | 99.3 ± 0.5 |
| par-2(it5ts) | 16.9 ± 3.7 | 0.8 ± 0.8 |
| par-2(lw32) | 1.7 ± 1.0b | ND |
| nos-3(q650) | 99.4 ± 0.3 | 75.2 ± 3.9 |
| spat-3(gk22) | 99.8 ± 0.0 | 98.7 ± 0.1 |
| rpn-10(tm1180) | 99.0 ± 0.1 | 99.2 ± 0.3 |
| rpn-10(tm1349) | 99.0 ± 0.2 | 97.3 ± 0.8 |
| nos-3(q650); par-2(it5ts) | 86.5 ± 2.0 | 53.6 ± 1.6 |
| nos-3(q650); par-2(lw32) | 54.6 ± 4.7b | ND |
| par-2(it5ts); spat-3(gk22) | 86.6 ± 2.2 | 22.9 ± 5.9 |
| par-2(lw32); spat-3(gk22) | 43.1 ± 6.8b | ND |
| rpn-10(tm1180); par-2(it5ts) | 62.2 ± 2.9 | 10.3 ± 1.8 |
| rpn-10(tm1349); par-2(it5ts) | 73.5 ± 2.7 | 8.8 ± 0.8 |
a
The value corresponds to the average percentage of embryos that hatched over the total number of embryos ± standard error of the mean over three independent assays; see materials and methods for details.
b
Viability for these particular assays was determined at 15° instead of 20° because par-2(lw32) animals also contain the mutation unc-45(e286ts), which confers an egg-laying-defective phenotype at higher temperatures.