TABLE 3.
RNAi clone | Gene | Embryonic viability in par-2(it5ts) (%)a | Embryonic viability in fbf-1(ok91); par-2(it5ts) (%)a |
---|---|---|---|
Vector | Negative control | 4.6 ± 3.1 | 12.9 ± 7.3 |
C32E12.2 | Negative control | 7.3 ± 7.5 | 19.8 ± 4.9 |
ZK20.5 | rpn-12b | 50.8 ± 11.5 | 64.1 ± 8.5 |
H12I13.a | fbf-1 | 7.5 ± 4.3 | 30.9 ± 5.8c |
F21H12.5 | fbf-2 | 5.2 ± 5.2 | 45.5 ± 14.6 |
Suppression was determined by feeding RNAi clones to animals at the L4 stage; see materials and methods for details.
The value corresponds to the average percentage of embryos that hatched over the total number of embryos ± standard deviation over three assays.
RNAi to rpn-12 was used as a positive control in this assay.
This fbf-1 RNAi clone might partially disrupt the function of fbf-2 since these two genes are 93% identical at the nucleic acid level.