Figure 6.—

Different GerB and GerF promoter deletions drive transient gfp reporter expression in various barley organs. All constructs were co-bombarded with Ubi∷uidA expression vector, pAHC25, as an internal control. (A) Coleoptile (1 dpi). Expression is strong (+++) under GerB (−1308/+15 bp) promoter. (B) Epicarp (gelatinous). Expression is strong (+++) under GerF (−1156/+15 bp) promoter. (C) Developing spike (elongating stage). Expression is strong (+++) under GerF (−1156/+15 bp) promoter. LE, lemma. (D) Developing spike (elongating stage). Expression is weak (+) under the GerF (−97/+15) promoter. (E) Lemma (elongating stage). Expression is strong (+++) under GerF (−356/+15 bp) promoter. (F) Lemma (elongating stage). Expression is weak (+) under the GerF (−97/+15) promoter, showing the effect of the −356/−97 fragment deletion. (G) Ubiquitin-driven uidA gene expression in lemma, same as in F. (H) No expression is detected in mature leaves under GerB (−1308/+15 bp) promoter. (I) Ubiquitin-driven uidA gene expression in mature leaves.