TABLE 1.
Primer pairs used for PCR screening
Primer pair (5′ to 3′) | Target gene (fragment size) | Target group | Annealing temp. [Mg+] |
---|---|---|---|
10F AGTTTGATCATGGCTCAGATTGa | 16S rRNA (∼1500 bp) | Most bacteria | 60° [1.5 mm] |
1507R TACCTTGTTACGACTTCACCCCAGa | |||
27F GAGAGTTTGATCCTGGCTCAGb | 16S rRNA (∼1470 bp) | Most bacteria | 55° [1.5 mm] |
1492R GGTTACCTTGTTACGACTTb | |||
559F CGTGCCAGCAGCCGCGGTAATACc | 16S-ITS-35R (>1000 bp) | Most bacteria (not Wolbachia) | 58° [1.5 mm] |
35R CCTTCATCGCCTCTGACTGCd | |||
10FF AGAGTTTGATCATGGCTCAGGATGc | 16S rRNA (∼1300 bp) | Cytophaga–Flavobacterium–Bacteroidetes | 58° [4.5 mm] |
1370R CGTATTCACCGGATCATGGCe | |||
63F GCCTAATACATGCAAGTCGAACd | 16S rRNA (∼450 bp) | Spiroplasma and several Gram-positive | 55° [1.5 mm] |
TKSSsp TAGCCGTGGCTTTCTGGTAAf | |||
WspF TGGTCCAATAAGTGATGAAGAAACTAGCTAg | wsp (∼600 bp) | Wolbachia | Touchdown 65–55° [1.5 mm] |
wspR AAAAATTAAACGCTACTCCAGCTTCTGCACg | |||
CLOf GCGGTGTAAAATGAGCGTGh | 16S rRNA (∼450 bp) | Cytophaga-like organism | 57° [1.5 mm] |
CLOr1 ACCTMTTCTTAACTCAAGCCTh | |||
LCO-1490 GGTCAACAAATCATAAAGATATTGGi | Mitochondrial COI | Most invertebrates | 45° [5 mm] |
HCO-2198 TAAACTTCAGGGTGACCAAAAAATCAi |