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. 2006 Sep;174(1):363–376. doi: 10.1534/genetics.106.058818

TABLE 1.

Primer pairs used for PCR screening

Primer pair (5′ to 3′) Target gene (fragment size) Target group Annealing temp. [Mg+]
10F AGTTTGATCATGGCTCAGATTGa 16S rRNA (∼1500 bp) Most bacteria 60° [1.5 mm]
1507R TACCTTGTTACGACTTCACCCCAGa
27F GAGAGTTTGATCCTGGCTCAGb 16S rRNA (∼1470 bp) Most bacteria 55° [1.5 mm]
1492R GGTTACCTTGTTACGACTTb
559F CGTGCCAGCAGCCGCGGTAATACc 16S-ITS-35R (>1000 bp) Most bacteria (not Wolbachia) 58° [1.5 mm]
35R CCTTCATCGCCTCTGACTGCd
10FF AGAGTTTGATCATGGCTCAGGATGc 16S rRNA (∼1300 bp) Cytophaga–Flavobacterium–Bacteroidetes 58° [4.5 mm]
1370R CGTATTCACCGGATCATGGCe
63F GCCTAATACATGCAAGTCGAACd 16S rRNA (∼450 bp) Spiroplasma and several Gram-positive 55° [1.5 mm]
TKSSsp TAGCCGTGGCTTTCTGGTAAf
WspF TGGTCCAATAAGTGATGAAGAAACTAGCTAg wsp (∼600 bp) Wolbachia Touchdown 65–55° [1.5 mm]
wspR AAAAATTAAACGCTACTCCAGCTTCTGCACg
CLOf GCGGTGTAAAATGAGCGTGh 16S rRNA (∼450 bp) Cytophaga-like organism 57° [1.5 mm]
CLOr1 ACCTMTTCTTAACTCAAGCCTh
LCO-1490 GGTCAACAAATCATAAAGATATTGGi Mitochondrial COI Most invertebrates 45° [5 mm]
HCO-2198 TAAACTTCAGGGTGACCAAAAAATCAi