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. 2006 Sep;174(1):363–376. doi: 10.1534/genetics.106.058818

TABLE 1.

Primer pairs used for PCR screening

Primer pair (5′ to 3′)	Target gene (fragment size)	Target group	Annealing temp. [Mg⁺]
10F AGTTTGATCATGGCTCAGATTG^a	16S rRNA (∼1500 bp)	Most bacteria	60° [1.5 mm]
1507R TACCTTGTTACGACTTCACCCCAG^a
27F GAGAGTTTGATCCTGGCTCAG^b	16S rRNA (∼1470 bp)	Most bacteria	55° [1.5 mm]
1492R GGTTACCTTGTTACGACTT^b
559F CGTGCCAGCAGCCGCGGTAATAC^c	16S-ITS-35R (>1000 bp)	Most bacteria (not Wolbachia)	58° [1.5 mm]
35R CCTTCATCGCCTCTGACTGC^d
10FF AGAGTTTGATCATGGCTCAGGATG^c	16S rRNA (∼1300 bp)	Cytophaga–Flavobacterium–Bacteroidetes	58° [4.5 mm]
1370R CGTATTCACCGGATCATGGC^e
63F GCCTAATACATGCAAGTCGAAC^d	16S rRNA (∼450 bp)	Spiroplasma and several Gram-positive	55° [1.5 mm]
TKSSsp TAGCCGTGGCTTTCTGGTAA^f
WspF TGGTCCAATAAGTGATGAAGAAACTAGCTA^g	wsp (∼600 bp)	Wolbachia	Touchdown 65–55° [1.5 mm]
wspR AAAAATTAAACGCTACTCCAGCTTCTGCAC^g
CLOf GCGGTGTAAAATGAGCGTG^h	16S rRNA (∼450 bp)	Cytophaga-like organism	57° [1.5 mm]
CLOr1 ACCTMTTCTTAACTCAAGCCT^h
LCO-1490 GGTCAACAAATCATAAAGATATTGGⁱ	Mitochondrial COI	Most invertebrates	45° [5 mm]
HCO-2198 TAAACTTCAGGGTGACCAAAAAATCAⁱ

^a

Munson et al. (1991).

^b

^c

Russell et al. (2003).

^d

Unpublished.

^e

Moran et al. (2003).

^f

Fukatsu and Nikoh (2000).

^g

Jeyaprakash and Hoy (2000).

^h

Weeks et al. (2003).

ⁱ

Folmer et al. (1994).