TABLE 3.

Species found positive for infection, with Wolbachia and Wolbachia haplotypes found

Species group or genus			Strains examined			Wolbachia infected
		Species		Ovaries	Whole flies	Total	Ovaries	Whole flies	Total	% strains infected	wsp haplotypes	16S haplotypes
cardini	D. arawakana	71	71	63	63	89	2 wWil; 56 wSpt
melanogaster	D. ananassae	3	17	20	3	7	10	50	7 wRi; 3 wSpt	3 wRia,b
melanogaster	D. baimaii	2	2	1	1	50	1 wBai (new)
melanogaster	D. bicornuta	2	2	2	2	100	2 wBic (new)
melanogaster	D. mauritiana	3	3	2	2	67	2 wNo
melanogaster	D. melanogaster	11	51	60	10	37	45	75	37 wMel	8 wMelc
melanogaster	D. nikananu	1	2	2	1	1	1	50	1 wNik (new)	1 wNika (new)
melanogaster	D. pseudoananassae	8	8	3	3	38	3 wPana	3 wPanaa
melanogaster	D. pseudotakahashii	2	2	1	1	50	1 wPse (new)
melanogaster	D. quadraria	1	1	1	1	100	1 wRi
melanogaster	D. sechellia	3	3	1	1	33	1 wHa
melanogaster	D. simulans	18	30	48	18	13	31	65	2 wMel; 27 wRi	16 wRia,b
melanogaster	takahashii subgroup	1	1	1	1	100	1 wTak (new)	1 wTakc (new)
melanogaster	D. teissieri	1	2	2	1	2	2	100	2 wSpt	1 wMelc
saltans	D. sturtevanti	7	7	2	2	29	2 wStv MI
willistoni	D. tropicalis	1	3	3	1	2	2	67	2 wWil
willistoni	D. willistoni	1	195	196	1	100	101	52	78 wWil	1 wWilc (new)
Scaptodrosophila	Scaptodrosophila stonei	2	2	1	1	50	1 wSto (new)
Scaptomyza	Scaptomyza pallida	1	1	1	1	100	1 wSpt
		Total:			434			271	62

^aPCR product obtained with both 27F–1492R and 10F–1507R primer pairs.

^bSequence is 1 bp different from another wRi strain (AY833061).

^cPCR product obtained with 27F–1492R primer pair only.