TABLE 1.
Genetic distances across a common a1–sh2 interval in three genetic backgrounds
Genetic background | No. isolateda | No. recoveredb | No. testedc | No. confirmedd | No. correct recombinantse | Population sizes | Genetic distances (cM)f |
---|---|---|---|---|---|---|---|
A632/LC | 229 | 138 | 130 | 130 | 229 | 320,718 | 0.143 ± 0.000667 |
Oh43/LC | 323 | 188 | 188 | 178 | 306 | 758,271 | 0.081 ± 0.00033 |
W64A/LC | 442 | 219 | 219 | 219 | 442 | 704,077 | 0.126 ± 0.000442 |
Number of putative colored shrunken recombinants isolated from cross 2 (materials and methods).
Number of putative recombinants successfully propagated.
Number of putative recombinants whose validity was tested (materials and methods).
Number of putative recombinants confirmed.
Number of correct recombinants = no. isolated × (no. confirmed/no. tested).
Genetic distance = 100 × no. correct recombinants × 2/population size. Because colorless round recombinants were not isolated, genetic distances were calculated by multiplying the number of colored shrunken recombinants by two. Standard errors were calculated according to the formula (pq/n)1/2. The genetic distances among genetic backgrounds varied significantly (P < 0.03).