TABLE 1.
Bqt2 is required for pairing of homologous chromosomes in meiotic prophase
Homologous chromosomes
|
Sister chromatids: | |||||
---|---|---|---|---|---|---|
% paired
|
% unpaired
|
% separated
|
||||
Parental genotypes | Expt. 1 | Expt. 2 | Expt. 1 | Expt. 2 | Expt. 1 | Expt. 2 |
mei4-B2 (GP4101) | 66 | 96 | 31 | 4 | 2 | 0 |
bqt2Δ mei4-B2 (GP5764) | 39 | 51 | 60 | 49 | 1 | 0 |
dhc1Δ mei4-B2 (GP5733) | 43 | 53 | 56 | 45 | 2 | 2 |
Cells were induced to mate and arrested in meiotic prophase. GFP dots, reflecting LacI-GFP bound to the lacO array at lys1, were counted in two experiments. In the first experiment 131, 99, and 129 zygotes were counted in wild-type, bqt2Δ, and dhc1Δ cells, respectively, and in the second 98, 102, and 113 were counted. One and two dots indicate paired and unpaired homologs, respectively. Three or four dots indicate separation of sister chromatids. The numbers are the percentage of zygotes in indicated classes for both experiments. In each experiment the fraction of paired homologs for both bqt2Δ and dhc1Δ was statistically different from that of wild type (P < 0.0005, contingency χ2-tests).