TABLE 2.

Pairing mutants demonstrate reduced allelic recombination and negative interference between a conversion and a crossover

	Recombinant frequency
Wild type		bqt2Δ		dhc1Δ
Interval (Chr)	Among total	Among Ade+a	Among totalb	Among Ade+a	Among totalb	Among Ade+a
lys3–met5 (I)	0.30	0.27	0.08**	0.10	0.14**	0.14
Clys3–met5c	0.92		1.18		1.02
leu1–pat1 (II)	0.37	0.43	0.16**	0.22*	0.14**	0.21*
Cleu1–pat1c	1.15		1.31*		1.46***
tps16–arg1 (III)	0.43	0.40	0.14**	0.31**	0.14**	0.34**
Ctps16–arg1c	0.93		2.29**		2.49**

Three or more independent crosses were performed for each interval. For the lys3–met5 interval the strains crossed were: wild type, GP13 × GP5570 (four crosses); bqt2Δ, GP5478 × GP5644 (four crosses); dhc1Δ, GP5572 × GP5642 (four crosses). For the leu1–pat1 interval: wild type, GP13 × GP5585 (three crosses); bqt2Δ, GP5478 × GP5584 (three crosses); dhc1Δ, GP5571 × GP5583 (three crosses). For the tps16–arg1 interval: wild type, GP5217 × GP5224 (five crosses) and GP5216 × GP5223 (two crosses); bqt2Δ, GP5219 × GP5226 (five crosses) and GP5218 × GP5225 (two crosses); dhc1Δ, GP5221 × GP5279 (three crosses) and GP5220 × GP5280 (one cross). Each frequency is based on the cumulative number of spore colonies, >500 colonies in each case.

^aStatistical significance relative to frequency among totals: *P < 0.02; **P < 0.0005.

^bStatistical significance relative to wild type: **P < 0.0005.

^cThe coefficient of coincidence, C, equals the observed frequency of crossovers among Ade⁺ spores divided by the frequency of crossovers among total spores. Statistically significant difference from C = 1: *P < 0.02; ***P < 0.0025; **P < 0.0005.