TABLE 3.
Pairing mutants demonstrate negative crossover interference
| Recombinant frequency
|
|||
|---|---|---|---|
| Interval (Chr) | Wild type | bqt2Δa | dhc1Δa |
| aim–tps16 (III) | 0.11 | 0.04** | 0.04** |
| tps16–arg1 (III) | 0.43 | 0.14** | 0.14** |
| Caim–tps16–arg1b | 1.2 | 2.5*** | 2.9 |
Three or more independent experiments were performed for each interval. Each frequency is based on >500 colonies analyzed. tps16–arg1 data are from Table 2.
a
Statistical significance relative to wild type: **P < 0.0005.
b
The coefficient of coincidence, C, equals the observed frequency of double crossovers divided by the product of the frequencies of the respective single crossovers. The numbers of double crossovers observed for wild type, bqt2Δ, and dhc1Δ were 62, 18, and 12, respectively. Statistically significant difference from C = 1: ***P < 0.05.