TABLE 4.
Telomere clustering, but not horsetail movement, limits ectopic recombination
| Recombinant frequency (× 105)
|
||||
|---|---|---|---|---|
| Locia | ΔDTb | wt | dhc1Δ | bqt2Δ |
| ade6 (allelic) | 0 | 530 ± 55 | 140 ± 8 | 120 ± 15 |
| ade6 × ChS28 | ∼1.34 Mb | 2.2 ± 0.5 | 2.4 ± 0.4 | 40 ± 6.2 |
| ade6 × zzz7 | ∼0.85 Mb | 8.9 ± 0.8 | 13 ± 1.5 | 32 ± 3.3 |
| ade6-Dup (SCE) | ∼5 kb | 1250 ± 160 | 1230 ± 120 | 1910 ± 160 |
Recombinant frequencies are the mean ± SEM for at least four experiments. wt, wild type.
a
The ade6-M26 and ade6-M210 alleles were used in the ade6 (allelic) and ade6 × ChS28 experiments. The ade6-M26 and ade6-469 alleles were used in the ade6 × zzz7 and ade6-Dup (SCE) experiments. The M210 and 469 alleles are 3 bp apart (Szankasi et al. 1988; G. Freyer, personal communication).
b
ΔDT is the difference between the distances of each locus from its nearest telomere (Figure 1B).