TABLE 1.
Rates of intrachromosomal recombination
| Substrate | Cell linea | Copy no.b | Cells tested (× 10−6) | HATR colonies | Recombination frequency (× 107)c | Recombination rate (× 108)d |
|---|---|---|---|---|---|---|
| pHYB12-8 | 12CE8 | 1 | 142 | 38 | 2.70 | 10.0 |
| 12CE8 | 1 | 139 | 6 | 0.43 | 3.8 | |
| 12CE10 | 2 | 169 | 70 | 2.10 | 6.6 | |
| 12CE10 | 2 | 221 | 8 | 0.41 | 1.9 | |
| Mean | 1.41 | 5.6 | ||||
| p1-8 | 1CE8 | 1 | 180 | 40 | 2.20 | 8.2 |
| 1CE8 | 1 | 175 | 49 | 2.80 | 9.8 | |
| 1CM1 | 1 | 189 | 16 | 0.85 | 4.1 | |
| Mean | 1.95 | 7.4 | ||||
| pHYB21A | D2 | 1 | 571 | 123 | 2.20 | 7.1 |
| K3 | 1 | 300 | 100 | 1.70 | 4.9 | |
| H3 | 2 | 382 | 36 | 0.47 | 1.8 | |
| Mean | 1.46 | 4.6 | ||||
| p1A | 10 | 1 | 292 | 28 | 0.94 | 3.9 |
| 5 | 1 | 277 | 13 | 0.46 | 2.5 | |
| Mean | 0.70 | 3.2 | ||||
| pHYB21A-28 | 1 | 1 | 256 | 14 | 0.55 | 2.8 |
| 2 | 1 | 210 | 3 | 0.14 | 1.4 | |
| Mean | 0.35 | 2.1 | ||||
| pHYB121 | 1 | 1 | 224 | 12 | 0.54 | 2.9 |
| 2 | 1 | 294 | 33 | 1.12 | 4.4 | |
| Mean | 0.83 | 3.7 |
a
For each cell line, data from independent fluctuation tests are presented in individual rows.
b
Number of copies of stably integrated recombination substrate in the particular cell line.
c
Calculated as the number of HATR colonies per number of cells tested divided by the number of copies of integrated substrate.
d
Recombination rate is expressed as number of recombination events per cell per generation per copy of integrated substrate.