Table 4.
Primers and fluorogenic probes for Real-time PCR
| Target/amplicon length | Specificity/sequence accession number | Primer and probe names | Primer/probe sequence | Lengh (bp) | Reference |
| Lectin 81 bp |
soybean specific gene (Accession number K00821) | TM-Lectin-F | 5'-tcc acc ccc atc cac att t-3' | 19 | 29 |
| TM-Lectin-R | 5'-ggc ata gaa ggt gaa gtt gaa gga-3' | 24 | |||
| Lectin-FAM | 5'-FAM-aac cgg tag cgt tgc cag ctt cg-TAMRA-3' | 23 | |||
| Invertase 79 bp |
maize specific gene (Accession number U16123) | ivr1-TM1 | 5'-tgg cgg acg acg act tgt-3' | 18 | 30 |
| ivr1-TM2 | 5'-aaa gtt tgg agg ctg ccg t-3' | 19 | |||
| ivr | 5'-VIC-cga gca gac cgc cgt gta ctt cta cc-TAMRA-3' | 26 | |||
| p35S 82 bp |
p35S promoter (Accession number V00141) | TM-35S-1 | 5'-gcc tct gcc gac agt ggt-3' | 18 | 29, 31 |
| TM-35S-2 | 5'-aag acg tgg ttg gaa cgt ctt c-3' | 22 | |||
| 35S | 5'-FAM-caa aga tgg acc ccc acc cac g-TAMRA-3' | 22 | |||
| tNOS 151 bp |
tNOS terminator (Accession number V00087) | tNOSF | 5'-gtc ttg cga tga tta tca tat aat ttc tg-3' | 29 | 32 |
| tNOSR | 5'-cgc tat att ttg ttt tct atc gcg t-3' | 25 | |||
| 5'-FAM aga tgg gtt ttt atg att aga gtc ccg caa-TAMRA- | |||||
| tNOSpro | 3' | 30 | |||
| GTS 40-3-2 soybean 83 bp |
construct specific (Accession number AB209952) | RRS-F | 5'-gcc atg ttg tta att tgt gcc at-3' | 23 | 29 |
| RRS-R | 5'-gaa gtt cat ttc att tgg aga gga c-3' | 25 | |||
| 5'-FAM-ctt gaa aga tct gct aga gtc agc ttg tca gcg- | |||||
| RRS | TAMRA-3' | 33 | |||
| MON810 maize 115 bp |
event specific (Accession number AF434709) | Mon810F1311 | 5'-cct tca taa cct tcg ccc g-3' | 19 | 33 |
| Mon810R1311 | 5'-aat aaa gtg aca gat agc tgg gca-3' | 24 | |||
| 5'-FAM-acg aag gac tct aac gtt taa cat cct ttg cca- | |||||
| Mon810pro1311 | TAMRA-3' | 33 | |||