Table 2.
In vitro antibacterial activity of purified phospholipase A2 enzymes from snake venoms.
| Phospholipase A2 enzymes (PLA2s) |
Snake species (Scientific name) | Mol. wt.(kDa) | Conc. (mg/mL) | Micro-organisms | |
| B. pseudomallei (Strain KHW) | B. pseudomallei (Strain TES) | ||||
| Crotoxin A (CA) | Crotalus durissus terrificus | 23.5 | 0.5 mg/mL | - | - |
| Crotoxin B (CB) | Crotalus durissus terrificus | 23.5 | 0.5 mg/mL | 24.8 ± 0.089 | 27.6 ± 0.133 |
| Ammodytoxin A | Vipera ammodytes ammodytes | 13.8 | 0.5 mg/mL | - | - |
| Mojave toxin | Crotalus scutulatus scutulatus | 23.5 | 0.5 mg/mL | - | - |
| β-Bungarotoxin | Bungarus multicinctus | 20.5 | 0.5 mg/mL | - | - |
| Taipoxin | Oxyuranus scutellatus scutellatus | 45.6 | 0.5 mg/mL | - | |
| Mulgatoxin | Pseudechis australis | 13.2 | 0.5 mg/mL | 20.5 ± 0.075 | 22.7 ± 0.117 |
| Daboiatoxin (DbTx) | Daboia russelli siamensis | 15.0 | 0.5 mg/mL | 24.8 ± 0.103 | 26.2 ± 0.121 |
| Bee venom PLA2 | Apis mellifera | 19.0 | 0.5 μg/mL | 18.3 ± 0.089 | 20.4 ± 0.075 |
*The values given represent a venom inhibition zone in mm, including the 7 mm diameter of the disc, after 24 h incubation. The bacterial inoculum per plate contained 3.2 × 108 cfu/mL forming units which were spread onto the TS agar surface with sterile cotton swap. Sterile paper discs (7 mm diameter) were placed onto the TS agar surface and 20 μL of enzymes (0.5 mg/ml concentration) added, Control (0); No activity (-).